Fig. 4

The increased expression of IFI6 in MSCs in leukemia niche promotes the proliferation of B-ALL cells. A Western blot was used to detect the expression levels of IFI6 in MSCs cultured alone and co-cultured with Nalm-6/RS4;11 for 72 h, mean ± SD. B The expression of IFI6 in normal tissues and tumor tissues through the analysis of GEPIA database. C The survival analysis of IFI6 in normal tissues and tumor tissues through GEPIA database. D and E The mRNA and protein levels of IFI6 in MSCs with control group (CON), empty group (EV) and up-regulated IFI6 group (LV-IFI6), mean ± SD. F Percentages of apoptotic Nalm-6 and RS4;11 cells co-cultured with blank, MSCs, MSCs-EV and MSCs-LV-IFI6 after vincristine sulfate treatments (2.5 μM for Nalm-6; 5 μM for RS4; 11) for 24 h, mean ± SD. G and H The numbers of migratory (G) and invasive (H) Nalm-6/RS4;11 cells co-cultured with blank, MSCs-CON, MSCs-EV and MSCs-LV-IFI6 groups after 24 h of cells incubation (40 × , scale bars, 100 µm), mean ± SD. I and J The numbers of cell proliferation of Nalm-6 and RS4;11 cells co-cultured with blank, MSCs, MSCs-EV and MSCs-LV-IFI6 groups after 24 h, 48 h, 72 h and 96 h of cells incubation, mean ± SEM, the “*” in the figure represents a significant difference between the MSCs-LV-IFI6 and MSCs-EV groups. Each value indicated three or more independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001