Fig. 1

MSCs in the leukemia niche exhibited diverse alterations. A The expression levels of POU5F1, SOX2 and NANOG in MSCs cultured alone group and MSCs co-cultured with Nalm-6/RS4;11 group for 72 h by qRT-PCR, n = 6 independent experiments. B The expression levels of RUNX1 and HOXB4 in MSCs cultured alone group and co-cultured with Nalm-6/RS4;11 group, n = 6. C and D The expression levels of the associated indicators of osteogenic (RUNX2, BGLAP) and adipogenic (ADIPOQ, PPAR-γ) differentiation in MSCs cultured alone group and co-cultured with Nalm-6/RS4;11 group, n = 6. E Representative images of Alizarin Red stained MSCs in cultured alone and co-culture with Nalm-6/RS4;11 group following 21 days of osteogenic induction and Oil Red O stain following 15 days of adipogenic induction (200 × , scale bars, 50 µm). F and G The expression levels of p53, p21 and p16 in MSCs cultured alone and co-cultured with Nalm-6/RS4;11, n = 6. H Senescence-associated β-galactosidase staining in MSCs cultured alone and co-cultured with Nalm-6/RS4;11 (200 × , scale bars, 50 µm), n = 3. I and J Percentages of apoptotic MSCs cultured alone and co-cultured with Nalm-6/RS4;11 for 72 h, n = 3. K Cell cycle arrest in MSCs cultured alone and co-cultured with Nalm-6/RS4;11 for 72 h, n = 3. Each value indicates the mean ± standard deviation (SD) of three or more independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001