Fig. 3

STING deficiency impaired macrophage polarization and pulmonary fibrosis. A The protein levels of STING after knocking out STING by CRISPR Cas9. B The proteins levels of STING signal pathway with or without STING in RAW264.7 cells. C The IFN-alpha and IFN-beta mRNA levels in RAW264.7 cells with or without STING after irradiation (n = 3). D The macrophages polarization of RAW264.7 cells with or without STING after irradiation (n = 3). E The protein levels of pulmonary fibrosis markers after irradiation in the co-culture system. Error bars represent mean ± SD. Statistical analyses were performed using two-tailed Students t-test or one-way ANOVA if more than two groups. *P < 0.05; **P < 0.01, ****P < 0.0001