Fig. 7
a Representative immunofluorescence images of CD31-positive microvessels (arrowheads) within the callus tissue of controls and sildenafil-treated mice at 2, 5 and 10 weeks after surgery. Scale bars: 25 μm. b CD31-positive microvessels/HPF within the callus tissue of controls (white bars, n = 8 at 2 and 5 weeks, n = 10 at 10 weeks) and sildenafil-treated animals (black bars, n = 8 at 2 and 5 weeks, n = 10 at 10 weeks) at 2, 5 and 10 weeks after surgery, as assessed by immunofluorescence analysis. c Representative immunohistochemical images of MPO-positive cells (arrowheads) within the callus tissue of controls and sildenafil-treated mice at 2, 5 and 10 weeks after surgery. Scale bars: 25 μm. d MPO-positive cells/HPF within the callus tissue of controls (white bars, n = 8 at 2 and 5 weeks, n = 10 at 10 weeks) and sildenafil-treated animals (black bars, n = 8 at 2 and 5 weeks, n = 10 at 10 weeks) at 2, 5 and 10 weeks after surgery, as assessed by immunohistochemical analysis. e Representative immunohistochemical images of CD68-positive cells (arrowheads) within the callus tissue of controls and sildenafil-treated mice at 2, 5 and 10 weeks after surgery. Scale bars: 25 μm. f CD68-positive cells/HPF within the callus tissue of controls (white bars, n = 8 at 2 and 5 weeks, n = 10 at 10 weeks) and sildenafil-treated animals (black bars, n = 8 at 2 and 5 weeks, n = 10 at 10 weeks) at 2, 5 and 10 weeks after surgery, as assessed by immunohistochemical analysis. Mean ± SEM; *p < 0.05 vs. control