Fig. 3

a Representative µCT-3D reconstructions of femora in controls and sildenafil-treated mice at 2, 5 and 10 weeks after surgery (yellow: total bone tissue within the upper panel; white: cortical bone, green: poorly mineralized bone, red: highly mineralized bone within the lower panel). Scale bar: 0.5 mm. b–d Poorly (PM) and highly mineralized (HM) bone volume (mm³) of the callus tissue of controls (white bars, n = 8 at 2 and 5 weeks, n = 10 at 10 weeks) and sildenafil-treated mice (black bars, n = 8 at 2 and 5 weeks, n = 10 at 10 weeks) at 2 (b), 5 (c) and 10 (d) weeks after surgery, as assessed by µCT analysis. Mean ± SEM; *p < 0.05 vs. control