Fig. 3

miR-125a-5p mimic drives differentiation of murine splenic CD4⁺T cells to Tregs. a miR-125a-5p expression in normal murine splenic CD4⁺ T cells transfected with miR-125a-5p mimic or mimic NC for 48 h by qRT-PCR (n = 5 per group, p < 0.001). b The proportion of CD25⁺ Foxp3⁺ T cells in normal murine splenic CD4⁺ T cells transfected with miR125a-5p mimic or mimic NC for 48 h by flow cytometry (n = 3 per group, p < 0.001). c The proportion of CD4⁺ IL17 ⁺ T cells in normal murine splenic CD4⁺ T cells transfected with miR125a-5p mimic or mimic NC for 48 h by flow cytometry (n = 3 per group, not significant). d ETS-1 expression in normal murine splenic CD4⁺ T cells transfected with miR125a-5p mimic or mimic NC for 48 h by qRT-PCR (n = 5 per group, p < 0.001). e IFN-γ expression in normal murine splenic CD4⁺ T cells transfected with miR125a-5p mimic or mimic NC for 48 h by qRT-PCR (n = 5 per group, p < 0.001). f STAT3 expression in normal murine splenic CD4⁺ T cells transfected with miR125a-5p mimic or mimic NC for 48 h by qRT-PCR (n = 5 per group, p < 0.001). Data are representative of three independent experiments (mean ± SEM). *P < 0.05, **P < 0.01, ***P < 0.001. Two-tailed unpaired Student’s t-test a, b, c, d, e and f miR microRNA; Tregs regulatory T cells; mimic NC mimic Negative Control; qRT-PCR quantitative real-time PCR; Foxp3 Fork head transcription factor3; IL interleukin; STAT3 Signal transducer and activator of transcription 3; ETS-1 E26 transformation specific-1; IFN-γ Interferon-γ