Fig. 3

HOTAIR increases ERK2 (MAPK1) expression by recruiting LSD1 to the promoter region of MAPK1. A ERK2 (MAPK1) and LSD1 expression in Hep3B and Huh7 CSCs with LSD1 silencing or HOTAIR silencing as examined by Western blot, normalized to GAPDH. B MAPK1 and HOTAIR expression in Hep3B and Huh7 CSCs with LSD1 silencing or HOTAIR silencing examined by RT-qPCR, normalized to GAPDH. *p < 0.05 compared with Hep3B and Huh7 CSCs treated with shCtl. C The expression of HOTAIR in Hep3B CSCs treated with shHOTAIR or combined with oe-LSD1 measured by RT-qPCR, normalized to GAPDH. *p < 0.05 compared with shCtl + oe-NC-treated Hep3B CSCs. D Western blot assay of ERK2 (MAPK1) and LSD1 proteins in Hep3B CSCs treated with shHOTAIR or combined with oe-LSD1, normalized to GAPDH. E The relative enrichment of LSD1 and H3K9me2 in the promoter region of MAPK1 in Hep3B CSCs with HOTAIR silencing assessed by ChIP, relative to Input. *p < 0.05 compared with shCtl-treated Hep3B CSCs. The experimental data were represented as mean ± standard deviation. Results between two groups were compared by unpaired t test. The data comparison between multiple groups was performed by one-way ANOVA with Tukey’s post-hoc test. Cellular experiments were repeated for three times