Fig. 1

Generation of the virtually stained H&E image and compartment building. a DAPI-stained image, b FITC-stained image used for calculating of c the virtually stained H&E image of the tissue sample. d The tissue areas in the tissue sections as classified by a pathologist: red–neoplastic tissue, green–non-neoplastic tissue. The blue area marks a region that was excluded from the analysis due to high autofluorescence or lost tissue during hybridisation. The derived representative binary tissue compartment (TC) e for the neoplastic and f for the non-neoplastic tissue