Fig. 4
From: IL-17A promotes tumorigenesis and upregulates PD-L1 expression in non-small cell lung cancer
IL-17A blocked autophagy in NSCLC cells via ROS/Nrf2/p62 pathway. A The intracellular superoxide anion level was detected by DHE. B The intracellular H2O2 level was measured by microplate method. C Immunofluorescence was used to detect the expression and localization of Nrf2 in cells. D Protein expression of Nrf2 and p62 at the total protein leve, cells were treated with or without 5 mM of N-acetylcysteine (NAC). E qPCR detection of HO1, NQO1 expression.*p < 0.05, **p < 0.01, ***p < 0.001, indicating statistically significant difference