Fig. 3

IL-17A blocked autophagy and increased the PD-L1 protein level in NSCLC cells. A PD-L1 expression in A549 and SPC-A-1 cells with different IL-17A expression levels were detected by immunofluorescence. Scale bar = 20 µm. B A549 and SPC-A-1 cells with different IL-17A expression levels were separately co-cultured with activated Jurkat T cells for 48 h. C Western blot analysis of protein expression of PD-L1 and autophagy-related proteins LC3 and p62. D Immunofluorescence staining of p62. scale bar = 20 µm. E Levels of LC3B I/II, p62 and PD-L1 protein were determined by Western blots in transfected A549 and SPC-A-1 treated with or without starvation, 10 mM of 3-methyladenine (3-MA), 1 nM of bafilomycin A1(Baf) or 20 nM of rapamycin (Rap) for 24 h. F Different expression of p62 and LC3 in IL-17A low and high expression groups. Bar = 50 μm