Fig. 2
From: IL-17A promotes tumorigenesis and upregulates PD-L1 expression in non-small cell lung cancer
IL-17A could promote NSCLC cell proliferation, migration, invasion and EMT. A Wound healing assay was performed to evaluate the migration capacity of A549 and SPC-A-1 after IL-17A knockdown or overexpression. Scale bar = 100 µm. B Detection of migration and invasion ability of A549 and SPC-A-1. Scale bar = 100 µm. C Plate colony assay of the transfected A549 and SPC-A-1 cells incubated for 3 weeks. D The expressions of EMT marker proteins N-cadherin, E-cadherin, Snail and Twist in A549 and SPC-A-1 cells with different IL-17A expression levels were detected by WB. E Immunofluorescence analysis of the expression of E-cadherin in A549 andSPC-A-1 with different IL-17A expression levels. Scale bar = 20 µm