Fig. 4

TDRKH-AS1 causes pyroptosis in HUVECs. A The relative expression pattern of DEGs induced by overexpression TDRKH-AS1 in HUVECs, including 968 up-regulated genes and 1062 down-regulated genes. B The enriched KEGG pathways of DEGs induced by overexpression TDRKH-AS1 in HUVECs. C Enrichment plots showing enrichment for NOD-like reception signaling pathway. D DEGs induced by overexpression TDRKH-AS1 are enriched with inflammatory/pyroptosis related genes. Error bars represent the standard deviation of the fraction, estimated using a bootstrapping method with 100 resampling. E The uptake of PKH26-labeled sEVs incorporates in HUVECs detected by confocal laser scanning microscopy. F The morphology of HUVECs is observed using scanning electronic microscopy. G The protein expression levels of NLRP3, cleaved-caspase-1, ASC and GSDMD-NT were measured by western blotting