Fig. 3
sEVs-derived TDRKH-AS1 induces preeclampsia-like symptoms in mice. A Schematic overview of the eight experimental groups. B The PKH26 red fluorescence using live animal imaging system. C The systolic blood pressure of the eight groups during pregnancy (n = 6, δ shows L-NAME treated mice vs. control, α shows AOPPs treated mice vs. PBS, β shows AOPPs-induced sEVs treated mice vs. sEVs control, γ shows LV-TDRKH-AS1 sEVs treated mice vs. sEVs LV-control, with.δδ, αα, ββ, γγp < 0.01). D The fetal weights of L-NAME, AOPPs, LV-TDRKH-AS1 sEVs and AOPPs-induced sEVs treated groups are significantly declined compared with those of the corresponding control groups. E Representative images of uterine horn, fetus and placenta in each group. F Representative images of labyrinth zone of placenta using H&E-staining. Dashed lines and asterisk indicate typical structural change (original magnification, × 200; scale bar = 100 µm). G–I The concentration of AOPPs, IL-1β and IL-18 are quantified in maternal plasma of each group by ELISA assay. The data are represented as the mean ± SD; **p < 0.01