Fig. 3

Mito-LND stimulates ROS generation and induces apoptosis in GBM cells. A, B After treatment with Mito-LND (2.5 µΜ) for 24 h, the levels of intracellular ROS in LN229 and U251 cells were measured by flow cytometry. The representative images showing ROS positive rate were showed. C Quantitative analysis of mean fluorescence intensity of DCFH-DA in GBM cells. D, E Cell apoptosis was analyzed by Flow cytometry in LN229 and U251 cells treated with different concentrations of Mito-LND. Quantitative analysis of the percentage of apoptosis in GBM cells. F Two GBM cells were treated with Mito-LND (0–2.5 µΜ) for 24 h. Caspase 3/7 activity were assessed by Caspase-Glo 3/7 activity assay. G The expression levels of Bax and Bcl-2 were analyzed after 24 h treatment of Mito-LND by Western blot. The data are presented as mean ± SD of three replicates (*P < 0.05, **P < 0.01)