Fig. 2

Mito-LND induces G0/G1 cell cycle arrest in GBM cells. A and C The cell cycle distribution of LN229 and U251 cells was analyzed via flow cytometry after treatment with indicated concentration of Mito-LND for 24 h, and the representative images are shown. B and D Quantitative analysis of cell cycle distribution. E, F LN229 and U251 cells were treated with Mito-LND (0-2.5 µΜ) for 24 h. The total protein was then extracted and examined through western blot analysis with the indicated antibodies. Values represent the mean ± SD, *, P < 0.05, **, P < 0.01