Fig. 4

Building better CAR-T cells. A Off-the-shelf/Universal CAR-T cells, Generation of universal CAR-T cells from an allogenic donor by deletion of MHC and TCRs using CRISPR/Cas-9 technology. B Tailor-made CAR-T cells for different TME. Multidomain and Armored CAR-T cells to tackle different tumor microenvironments to stimulate CAR-T cell growth. C Molecular Switches to control CAR-T cell cytotoxicity, Programming of CAR-T cells with degron (degradation) sequence and iCasp9 (Inducible caspase 9) to induce their destruction upon life-threatening toxicity in treated patients. D Alternate to ScFv. Potential alternative to bulky ScFv like DARPin based CARs and Fn3 recognition domain CAR-T cells. E Making CAR-T cells financially viable therapeutic modality. Reduction cost of production and introduction of universal CAR-T cells, which can be produced in bulk. F Novel CAR-T cell based combination therapies. Combination CAR-T cell therapy with novel immunomodulatory agents will help in achieving a durable therapeutic response in cancer patients