Fig. 6

MFAP5 + fibroblasts modulate the phenotype of C1QC + macrophages through the IL34/CSF1R signal pathway. A The hierarchy plots of CSF signaling pathway network indicate the regulation of MFAP5 + fibroblasts and FABP5 + fibroblasts on myeloid cells in normal or tumor sites. B Violin plot of IL34, CSF1, and CSF1R expression of tumor-specific fibroblasts and myeloid cells in distinct tissues (normal or tumor). C Heatmap shows dominant senders, receivers, mediators and influencers in CSF signals of tumor inferred by network centrality score. D Bar plot shows the contribution of all L-R pairs in CSF pathway within the normal tissue. E Spatial images localize the expression of IL34 and CSF1R which suggest that the co-localization of IL34 and CSF1R in CRC slices. F Heat map of M1/M2 phenotype scores of three identified tumor-associated macrophage subtypes. G Immunohistochemical double-staining of MFAP5/CD163 and IL-34/CSF1R using continuous slides of CRC tissues (×20, 50 μm)