Fig. 3

Multi-omics analyses uncover the cell-to-cell communications between MFAP5 + fibroblasts and macrophages. A Spatial images show the tissue architecture of CRC inferred by unsupervised clustering method. B Heatmap of immune cells and fibroblasts infiltration level scored by ssGSEA algorithm. C Gene expression of CD3D, CD8A, CD19, MS4A1, GZMA, GNLY, CD68, CD163 as well as MFAP5 + fibroblasts (top 20 DEGs identified in scRNA-seq) in spatial organization of patient 2 (P2), red boxes mark the representative tumor region of CRC. D Scatter plots show the positive correlation between MFAP5 + fibroblasts and macrophages in COAD and READ cohorts. E Correlation analysis of MFAP5 and tumor-infiltrating immune cells shows the highest correlation between MFAP5 and macrophages calculated by TIMER tool