Fig. 6

Transcriptional signatures and functional properties of our engineered T cells. A Heat map of differences in gene expression in the whole transcriptome after 24 h of co-culture of CR, CAR, CR + CAR, and NTD T cells and tumor cells. B Volcano plots show differences in transcriptional gene expression between CR and CR + CAR group T cells and tumor cells after 24 h of co-culture. C Volcano plot of volcanic differences in transcriptional gene expression between T cells in the CAR and CR + CAR groups and tumor cells after 24 h of co-culture; D KEGG pathway analysis of CR and CR + CAR group T cells expression difference gene mainly involved in the signaling pathway; E KEGG pathway analyzes the signaling pathways in which T cells in the CAR and CR + CAR groups express differences in genes. F Gene set enrichment analysis of IL6/JAK/STAT3 and Glycolysis signaling gene sets in CAR and CR + CAR T cells from RNA-seq, the heatmap of the top 20 up-regulated genes in the CAR group was listed in each gene set. G Gene set enrichment analysis of Oxidative Phosphorylation and Fatty Acid Metabolism signaling gene sets in CAR and CR + CAR T cells from RNA-seq, the heatmap of the top 20 down-regulated genes in the CAR group was listed in each gene set. (n = 3 biological replicates). All data are presented as the mean ± SEM of triplicate experiments. *p < 0.05, **p < 0.01, ***p < 0.001, P > 0.05 labeled as N.S was considered no significance