Fig. 5

Validating in other TCGA patients with different tumors and colorectal cell lines. a, b The top five candidate gene combinations with AUC values in TCGA breast cancer patients (a), and ROC curve of the best prognostic model (BRCA, n = 38) (b). c, d The top 5 combinations in lung adenocarcinoma patients (c) and the best ROC curve (LUAD, n = 36) (d). e, f The top 5 combinations in cervical cancer patients (e) and the best ROC curve (CESC, n = 20) (f). g HT-29 and HCT 116 cells were used for clonogenic formation assay, and the results were displayed through a linear quadratic model. The experiment was repeated three times independently, and statistics were performed by two-tailed ANOVA test. **P ≤ 0.01; ***P ≤ 0.001. h Detecting basic mRNA expression of four key genes in two kinds of cells by RT-qPCR. Each sample was repeated three times independently and standardized by GAPDH. ***P ≤ 0.001