Fig. 4

Validating the correlation between clinical trials and radiation resistance gene panel by weighted gene co-expression network. a Scale independence and mean connectivity were utilized to pick up the soft threshold of WGCNA (power = 9). b The top 10,000 genes of CO/READ patients who received radiotherapy were included to build cluster tree. Similar genes were clustered in gene modules represented by colors (dynamic tree cut), and similar modules were fused to form “merged dynamic”. c Heatmap matrix was used to indicate the relationship between gene modules and clinical trials. Gene modules were listed in rows, while PFS and risk score were listed in column. Correlation and P.value were marked in each grid. d Gene modules corresponding to the four genes, and a brief overview of gene functions. e–h Dot chart described pathways enriched by GO analysis. LGR5, KCNN4, TNS4 and CENPH were classified into greenyellow e, salmon f, brown g and grey h modules respectively. Gene counts expressed by dot size and P.value by color were listed next to each graph