Fig. 1

Genetic profiling and overall design to explore radiation resistance and tumor repopulation. a Volcano plot of radiation resistant cells vs. control cells in GSE97543. |Log2 fold change|≥ 1 and P.value ≤ 0.05 were set as cut-off values. Red dots were recognized as upregulated genes, while blue as down-regulated ones in DEGs analysis. b 710 upregulated genes were imported into Metascape website for pathway enrichment analysis. c Volcano plot of tumor vs. normal tissues in TCGA-CO/READ database. Cut-off values and colors were same as before. d Venn diagram showed the intersection of the upregulated genes in the TCGA and GEO databases. 252 co-upregulated genes were spotted. e Details of the data included in this study. Resistant rectal adenocarcinoma cells and control cells included in GEO database were both treated by 4 Gy radiation. TCGA database contained colon and rectal adenocarcinoma patients. f Visualization of the overall experimental design. 252 co-upregulated genes were used to screen key genes to form a gene panel