Fig. 6

TIM enhances the transcription of PD-L1 through c-Myc. a The expression level of PD-L1 after knockdown of c-Myc in the MCF-7 cells and the BT-20 cells. b Relative mRNA levels of TIM, c-Myc, and PD-L1 after knockdown of c-Myc in the MCF-7 cells and the BT-20 cells. c The interaction of TIMELESS and c-Myc detected by co-immunoprecipitation in the MCF-7 cells and the BT-20 cells. d Immunofluorescence staining of TIM (pseudo-color: red) and c-Myc (pseudo-color: green) in MCF-7 cells and the BT-20 cells. Hoechst: nuclear counterstaining (pseudo-color: blue). The scale bar is 25 μm. e The expression level of PD-L1 after knockdown of c-Myc in both MCF-7 cells and BT-20 cells with TIM overexpression. Significance was tested using Mann–Whitney U-test: ns, p-value no significance; ***p-value < 0.001