Fig. 4

TIM regulates PD-L1 expression of breast cancer cells in vitro and in vivo. a The mRNA expression level of PD-L1 in breast cancer TIM knockdown cells. The mRNA expression level was analyzed by RT-qPCR. β-actin was used as an internal reference. b Western blot analyses of TIM and PD-L1 in breast cancer TIM knockdown cells. β-actin was used as an internal reference. c Representative flow cytometric plot of PD-L1 membrane expression level in breast cancer TIM knockdown cells. d The mRNA expression level of PD-L1 in breast cancer TIM overexpression cells. The mRNA expression level was analyzed by RT-qPCR. β-actin was used as an internal reference. e Western blot analyses of TIM and PD-L1 in breast cancer TIM overexpression cells. β-actin was used as an internal reference. f Representative flow cytometric plot of PD-L1 membrane expression level in breast cancer TIM overexpression cells. g Western blot analyses of TIM and PD-L1 in the syngeneic tumor mice. β-actin was used as an internal reference. Significance was tested using Mann–Whitney U-test: *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001