Fig. 7

AdipoR1 and AdipoR2 regulates of breast cancer progression. A Scatter plot showing the CRISPR gene dependency of ADIPOR1 and ADIPOR2 in breast cancer cell lines. B Western blot analysis showing the effect of knockdown by three shRNA sequences in cell line MDA-MB-231. C Quantitative analysis showing the proliferation of cell line MDA-MB-231, MCF7 and ZR-751 after knockdown of AdipoR1 and AdipoR2. D Flow cytometry results and quantitative analysis showing the cell death level of cell line MDA-MB-231 after knockdown of AdipoR1 and AdipoR2. E Transwell assay result and quantitative analysis showing the invasion ability of cell line MDA-MB-231 after knockdown of AdipoR1 and AdipoR2. The number of cells stained by crystal violet under the view of microscope represents the invasiveness of cancer cells. F Representative immunohistochemical images of AdipoR1 and AdipoR2 in breast cancer patients. G Kaplan–Meier survival curve for breast cancer patients in AdipoR1/2 positive or negative group. P value was calculated with log‑rank test. Log‑rank p value < 0.05 was considered as statistically significant