Fig. 3

Effects of sevoflurane on electrical resistance and proinflammatory cytokines levels in conditioned medium of mouse lung epithelial (MLE-12) cell monolayer. a Electrical resistance of a monolayer of MLE-12 cells was measured at a frequency at 4000 Hz by electric cell-substrate impedance sensing (ECIS) in untreated cells (Medium) or in cells treated for 24 h with cytomix alone (Cyto), sevoflurane alone (Sevo) or with cytomix and sevoflurane (Cyto + Sevo). Results are shown as medians with interquartile ranges (n = 35–40 per group and per timepoint). b Medium levels of Chemokine C-X-C motif ligand-1(CXCL-1), c Interleukin 6(IL-6) and d Tumor necrosis factor-alpha (TNF-α) at 6 h in identical conditions. Results are shown as medians with interquartile ranges (n = 3 per group). Two-way ANOVA test was performed, with post-hoc comparisons if ANOVA results showed significance (compared to the Medium group: *p < 0.05; **p < 0.01; ***p < 10^–3; ****p < 10^–4; compared to the Cyto group: ^##p < 0.01; ^###p < 10^–3; ^####p < 10^–4)