Fig. 2

Lung junction proteins zonula occludens (ZO)-1 and E-cadherin and lung myosin light chain 2 (Ser19) phosphorylation (pMLC) in vivo. Immunostaining of lung a ZO-1 and b E-cadherin in lung tissues from uninjured (Sham), acid-injured (HCl), and acid-injured mice treated with sevoflurane (HCl + Sevo) on day 1 after injury. Tissues were fixed, permeabilized, and stained with ZO-1 and E-cadherin antibodies, followed by A488 secondary antibodies and Hoechst staining. All images were acquired by a fluorescent microscope with a 20× objective. a ZO-1 protein is red-stained, and the cell nucleus is blue-stained. b E-cadherin protein is red-stained, and the cell nucleus is blue-stained. Scale bar: 50 μm. c Western blots of total myosin light chain (MLC) and phosphorylated myosin light chain 2 (Ser19) (pMLC) in lung of uninjured (Sham), acid-injured (HCl), and acid-injured mice treated with sevoflurane (HCl + Sevo) from day 0 to day 4 after injury. d Protein expression levels were quantified and standardized by GAPDH protein level, and pMLC (Ser 19) levels were additionally standardized by total MLC level, expressed as ratios to those in Sham animals, and reported as box and whisker plots with medians and interquartile ranges (n = 4–6 per group). Two-way ANOVA test was performed, and no significance was found