Fig. 5

Upregulated miR-185-5p promotes the malignancy of CRC. A Detection of miR-185-5p expression in the SW480 cells transfected with miR-185-5p mimics and in the RKO cells transfected with miR-185-5p inhibitors using RT-qPCR. B An increase in the expression of miR-185-5p in the SW480 cells and a knockdown of the expression of miR-185-5p in the RKO cells affected their proliferation (CCK-8 assay). C An increase in the expression of miR-185-5p enhances the migration ability of the SW480 cells. D An Inhibition of the expression of miR-185-5p relieved the migration ability of the RKO cells. E Construction of the SW480 cells that stably overexpressed miR-185-5p. F Tumor volume and weight in the SW480-185OE group were larger than those in the SW480-Con group. G Comparison of the tumors taken from 185OE and Con groups. H Ki67-positive cells in tumors detected using immunohistochemical staining. I Tunel-positive cells in tumors detected using immunofluorescence staining. *, P < 0.05; **, P < 0.01; ***, P < 0.001. *, P < 0.05; **, P < 0.01; ***, P < 0.001