Downregulation of Claudin5 promotes malignant progression and radioresistance through Beclin1-mediated autophagy in esophageal squamous cell carcinoma

Table 2 Radiobiological parameters of ESCC cells (associated with Fig. 5g)

Cell lines	Group	D₀ (Gy)	D_q (Gy)	N	SF2 (%)
TE1	si-Claudin5#2 + DMSO	3.58 ± 0.17	4.85 ± 0.14	2.35 ± 0.22	85.00 ± 1.20
	si-Claudin5#2 + 3-MA	2.69 ± 0.20^a^,*	2.39 ± 0.24^a^,*	1.89 ± 0.12^a^,*	70.00 ± 1.8^a^,*
	si-Claudin5#2 + sh-NC	3.29 ± 0.16	3.82 ± 0.21	2.16 ± 0.15	82.12 ± 2.50
	si-Claudin5#2 + sh-Beclin1	2.98 ± 0.13^b,*	2.39 ± 0.14^b,*	1.80 ± 0.13^b,*	72.11 ± 1.60^b,*

Radiobiological parameters (D₀, D_q, N, and SF2) were calculated from the single-hit multitarget model (SF = 1 − (1 − e^ [− kD]) ^N) using GraphPad Prism Version 9.5.1 software. Data represent three independent experiments (mean ± SD)
D₀, final slope; D_q, quasi-threshold dose; N, extrapolation number; SF2, survival fraction of 2 Gy
*p < 0.05, two-tailed t-test, unpaired
^aSi-Claudin5#2 + DMSO versus si-Claudin5#2 + 3-MA
^bSi-Claudin5#2 + sh-NC versus si-Claudin5#2 + sh-Beclin1

ISSN: 1479-5876