Downregulation of Claudin5 promotes malignant progression and radioresistance through Beclin1-mediated autophagy in esophageal squamous cell carcinoma

Table 1 Radiobiological parameters of ESCC cells (associated with Fig. 3)

Cell lines	Group	D₀ (Gy)	D_q (Gy)	N	SF2 (%)
TE1	si-NC	2.98 ± 0.12	2.39 ± 0.12	1.80 ± 0.14	72.11 ± 1.60
	si-Claudin5#2	3.29 ± 0.14^a^,*	3.82 ± 0.14^a^,*	2.16 ± 0.16^a^,*	82.12 ± 2.50^a^,*
	si-Claudin5#3	3.20 ± 0.13^b,*	5.53 ± 0.23^b,*	2.73 ± 0.15^b,*	87.60 ± 1.20^b,*
KYSE150	Vector	2.76 ± 0.12	3.62 ± 0.20	2.31 ± 0.15	77.40 ± 1.80
KYSE150	Claudin5	2.25 ± 0.16^c,*	2.13 ± 0.17^c,*	1.95 ± 0.19^c,*	64.01 ± 1.20^c,*

Radiobiological parameters (D₀, D_q, N, and SF2) were calculated from the single-hit multitarget model (SF = 1 − (1 − e^ [− kD])^N) using GraphPad Prism Version 9.5.1 software. Data represent three independent experiments (mean ± SD)
D₀, final slope; D_q, quasi-threshold dose; N, extrapolation number; SF2, survival fraction of 2 Gy
*p < 0.05, two-tailed t-test, unpaired
^aSi-Claudin5#2 versus si-NC
^bSi-Claudin5#3 versus si-NC
^cClaudin5 versus Vector

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