Fig. 3

Downregulation of Claudin5 reduced radiosensitivity of ESCC cells. Clonogenic survival assay was used to evaluate the effects of Claudin5 on ESCC cells radiosensitivity. A, C Representative images of colony formation after irradiation. B, D Survival curves of ESCC cells treated with irradiation. The survival curves were plotted using GraphPad Prism Version 9.5.1 according to the single-hit multitarget model (SF = 1 − (1 − e^ [− kD])^N). Associated radiobiological parameters (D₀, D_q, N, and SF2) were calculated and shown in Table 1. The statistical difference was assessed with one-way ANOVA followed by Dunnett tests in B; and the two-tailed unpaired Student t test in D. Error bars show the SD from three independent experiments. **p < 0.01