Fig. 6

Hsa_circ_0002348 regulated BAK1 expression by competing for miR-126-3p. A Venn diagram showing the identification of miR-126-3p targeted genes, predicted from the public databases; the binding sites of miR-126-3p and BAK1 in the right; B luciferase reporter activity of BAK1 3′UTR in HTR8 cells co-transfected with miR-126-3p mimics and NC mimics; C qRT-PCR analysis of BAK1 expression levels in HTR8 cells transfected with hsa_circ_0002348 knockdown; D qRT-PCR analysis of BAK1 expression levels in HTR8 cells transfected with miR-126-3p mimics; E qRT-PCR analysis of BAK1 expression levels in HTR8 cells treated with hsa_circ_0002348 overexpression and miR-126-3p mimics; F western blotting analysis showing the expression of BAK1 compared with Tubulin as internal control; the quantitative analysis of band intensity performed with Image J (right); G qRT-PCR analysis of hsa_circ_0002348 overexpression in HTR8 cells transfected with miR-126-3p mimics (left) and hsa_circ_0002348 overexpression co-transfected with or without miR-126-3p mimics (right); H representative IHC image of BAK1 expression in the pregnant mice’s placentas of each group (n = 3/group; left); the semi-quantitative analysis of BAK1 staining performed with Image J (right); I representative IHC image of BAK1 in the placentas of the preeclampsia patients (n = 3) and normal healthy controls (n = 3; left); the quantitative analysis performed with Image J (right); NC negative control, Ctrl control; circ_0002348 representing hsa_circ_0002348; circ_0002348 over representing hsa_circ_0002348 overexpression; the data presented as mean ± SEM; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001