Fig. 2

Effects of CD40Apt on TGF-β-induced orbital fibroblast activation. A Orbital fibroblasts were challenged with 10 ng/ml TGF-β for 48 h, treated with PBS, non-specific control aptamer, or different concentrations of CD40Apt (750/500/250/100 nM), and examined for cell viability using CCK-8 assay. Then, orbital fibroblasts were challenged with 10 ng/ml TGF-β for 48 h, treated with PBS, non-specific control aptamer, or 500 nM CD40Apt for 48 h, and examined for the levels of α-SMA using Immunofluorescent staining (B); the mRNA expression of α-SMA, collagen I, Timp-1, and vimentin using qRT-PCR (C); the protein levels of α-SMA, collagen I, Timp-1, and vimentin using Immunoblotting (D); the levels of collagen I in supernatant using ELISA (E)