Fig. 4

Reduction of SIRT1 resists the progression of EMs in METTL3-depleted ESCs. SIRT1 exhaustion rescued the ability of proliferation by EdU assay A, the ability of migration by wound healing assay B, the level of cellular senescence by SA-β-Gal staining C, the ability of invasion by Transwell assay in METTL3 depletion ESCs. E The protein level of METTL3, SIRT1, and senescence biomarkers in ESCs transfect with si-NC, si-METTL3, si-SIRT1, and si-METTL3 + si-SIRT1 by western blotting, using GAPDH as an internal control. F SIRT1 knockdown reduced the ability of viability induced by METTL3 knockdown. All above result were shown in means ± SD, ns, p ≥ 0.05; *p < 0.05; **p < 0.01; ***p < 0.001