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Fig. 8 | Journal of Translational Medicine

Fig. 8

From: LINC00963-FOSB-mediated transcription activation of UBE3C enhances radioresistance of breast cancer cells by inducing ubiquitination-dependent protein degradation of TP73

Fig. 8

LINC00963 binds to FOSB to activate UBE3C expression. A RNAs binding to UBE3C and proteins binding to LINC00963 predicted by CatRapidomics analysis; B binding between LINC00963 and FOSB examined by biotin-LINC00963-based RNA pull-down assay; C, bidning between LINC00963 and FOSB validated by RIP-qPCR assay; D binding between LINC00963 and FOSB validated by MDA-MB-231 and SK-BR-3 cells; E enrichment of UBE3C promoter fragments in the complexes pulled down by biotin-LINC00963 examined by qPCR analysis; F, G RT-qPCR and WB assays for the mRNA and protein levels of UBE3C in MDA-MB-231 and SK-BR-3 cells overexpressing LINC00963; H nuclear translocation of FOSB in LINC00963-overexpressing MDA-MB-231 and SK-BR-3 cells determined by immunofluorescence staining. Data are expressed as the mean ± SD. Three repetitions were performed. Differences were analyzed by two-way ANOVA. **p < 0.01, ***p < 0.001

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Journal of Translational Medicine

ISSN: 1479-5876

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