Fig. 4

H19 delivered by CSCs-EVs enhances the malignant behaviors of NSCCs and upregulates the expression of stemness marker proteins. A H19 expression in NSCCs, CSCs and their EVs detected by RT-qPCR. B H19 expression in NSCCs co-cultured with CSCs-EVs detected by RT-qPCR. C Knockdown efficiency of H19 gene in CSCs detected by RT-qPCR. D H19 expression in NSCCs co-cultured with CSCs-EVs-sh-H19 detected by RT-qPCR. E Viability of NSCCs co-cultured with CSCs-EVs-sh-H19 determined by CCK-8 assay. F Migration and invasion of NSCCs co-cultured with CSCs-EVs-sh-H19 determined by Transwell assay. G Western blot of the stemness marker proteins CD44, CD24, CD133, and Lgr5 in NSCCs co-cultured with CSCs-EVs-sh-H19. Cell experiments were conducted three times independently. *p < 0.05, **p < 0.01, ***p < 0.001