Fig. 2

APE1 temporally affects the formation of DSBs following TBHP Exposure. a HeLa NC and shAPE1 cell lines were treated with 100 μM TBHP for 1 h (treatment regime for TBHP was used throughout the study unless stated otherwise) and allowed to recover for 1, 4 and 36 h, the distribution of γ-H2AX foci were assessed by IF. b the data of a were normalized to the proportion of cells with > 10 foci after mock or TBHP treatment. c–e Residual SSB and DSB levels of HeLa NC and shAPE1 at 1 and 24 h post-TBHP treatment were evaluated using alkaline and neutral comet assays. The representative images are shown in c. d–e Tail moment values were calculated and plotted via a distribution dot plot for > 100 cells. The data were presented as the mean ± SEM from three independent experiments. The p-values were determined using an unpaired Student’s t-test (****p < 0.0001)