Fig. 5

AI662270 regulates its direct target Abca1. A Representative examples of cross sections from aortic sinus labeled for Abca1, and Abca1 fluorescence intensity was analyzed (Scale bar = 20 µm, n = 4 mice); **p < 0.01 vs. mice in CD; ##p < 0.01 vs. Lv-null treated mice in HFD. B Transfection efficacy was measured using qRT-PCR (n = 5); **p < 0.01 vs. NC-P. C, D Relative mRNA and protein expression of Abca1 were determined derived from AI662270 overexpression in peritoneal macrophages treated with ox-LDL (100 µg/mL). n = 6 in (C) and n = 5 in (D); **p < 0.01 vs. Ctr; ##p < 0.01 vs. + NC-P. E Transfection efficacy was detected using qRT-PCR (n = 6); *p < 0.05 or **p < 0.01 vs. + siNC. F, G Protein (F) (n = 3) and mRNA (G) (n = 6) expression of Abca1 were analyzed. **p < 0.01 vs. + siNC. “ + ” represents peritoneal macrophages cotreatment with ox-LDL (100 µg/mL) in (C, D, F, G). H RNA-interacting protein immunoprecipitation (RIP) analysis for AI662270:Abca1 interaction (n = 3); **p < 0.01 vs. anti-IgG. I RNA pulldown of AI662270 dragged down an appreciable quantity of Abca1 (n = 3). **p < 0.01, ##p < 0.01 vs. AI662270-1/2. Data are expressed as mean ± SEM