Fig. 6

Analysis of CD8⁺ T cell transition states in HD and WM samples. A Pseudotime-ordered analysis of CD8⁺ T cells from HD (bottom) and WM (top) samples. CD8⁺ T cell subtypes are labeled by colors. B The distribution of CD8⁺ subtypes during the transition (divided into 4 phases), along with the pseudotime. Subtypes are labeled by colors (upper panel). Heatmap showing the dynamic changes in gene expression (divided into 3 groups) along the pseudotime, and their enriched pathways by Ingenuity Pathway Analysis (IPA, lower panel). C Two-dimensional plots showing the cell scores of genes related to naïve, cytotoxic, and exhausted CD8⁺ T cells along with the pseudotime (divided into 4 phases). Cells scores are labeled by line colors. D Density line plot showing the cell distribution in low/high-tumor-infiltration WM patients along with the pseudotime, by state (top). Histogram displaying the cell proportion of 4-phase CD8⁺ T cells in WM of 2 groups (top center, Chi-square test between low/high-tumor-infiltration WM patients, P < 0.001). 2D graph of the pseudotime-ordered CD8⁺ T cells, from WM of 2 groups (bottom). E Sankey diagram showing the proportions of 4-phase CD8⁺ T cells in low/high-tumor-infiltration WM patients