Fig. 3

Inhibition ofAnxa2improved hepatocyte pyroptosis and liver fibrosis in NASH mice. (A) WB for detecting the inhibitory effects from Lv-shAnxa2 on the expression of ANXA2 and pyroptosis related proteins (including ASC, pro-Caspase1, C-Caspase1, GSDMD, and GSDMD-N) in liver tissues of NASH mice. (B) HE staining for detecting the pathological changes of NASH liver. (C) Masson staining for detecting the effects of Anxa2 inhibition on liver fibrosis. (D) Immunohistochemical staining for detecting the effects from Anxa2 inhibition on α-SMA expression. (E) The serum levels of ALT, AST, IL-1β, and CRP. Eight mice were included in each group. *: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001