Fig. 8
Expression and function of ZC3H13 in cervical cancer and the effects of rapamycin on cell phenotypes. A The qPCR analyses showed that ZC3H13 was significantly downregulated in cervical cancer tissues (P = 0.0019). B The IHC analysis showed that ZC3H13 was localized in the nucleus and was expressed at low levels in cervical cancer tissues (× 20) (scale bar: 50 μm). Gray level difference analysis showed that the IOD of ZC3H13 in normal cervical tissues was significantly higher than that in cervical cancer tissues. C The WB analysis demonstrated the low expression of ZC3H13 in cervical cancer tissues. D The CCK8 assay showed that the proliferation ability of HeLa and SiHa cells was significantly enhanced after ZC3H13 knockdown. E The CCK8 assay showed that the proliferation ability of HeLa and SiHa cells was significantly reduced after rapamycin treatment. F The wound-healing assay showed that ZC3H13 knockdown significantly enhanced the invasion abilities of the HeLa and SiHa cells. The wound-healing assay showed that the invasion ability of HeLa and SiHa cells was significantly reduced after rapamycin treatment. G The Transwell experiments showed that ZC3H13 knockdown significantly enhanced the migration abilities of the cells. The Transwell assay showed that the migration ability of HeLa and SiHa cells was significantly reduced after rapamycin treatment. H The m6A ELISA analyses showed that the m6A levels decreased significantly after the ZC3H13 knockdown. I The m6A ELISA analyses showed that the m6A levels increased significantly in both HeLa and SiHa cells after rapamycin treatment. All experiments were repeated at least three times (*P < 0.05, **P < 0.01, ***P < 0.001)