Fig. 5

Nutraceutical polyphenols extract decreases intracellular neutral lipid in McR-Rh7777. Rat hepatoma cell line McARh-7777 was cultured in 2D and incubated with 50 µM of oleic acid and different concentrations of nutraceutical (25–12.5 µg/mL) or placebo in regular medium without FBS for 24 h. Intracellular lipid content was measured by Oil red-O staining, and representative images were acquired at 40× magnification (A). ORO area quantified by Image J (B) and showed a significant decreased of intrahepatic lipids content. Data shown as mean ± SD in all groups. Statistical analysis: *p < 0.05; **p < 0.01 (Student t Test) vs 0; ^#< 0.05 ^##p < 0.01 (Student t test) vs. placebo