Fig. 10

Validation of lncRNA-AC079061.1/VIPR1 axis. A, B, C The expression of lncRNA- AC079061.1, hsa-miR-765 and VIPR1 in the HCC cell lines and normal live cells. D, E Confirmed the proliferation of lncRNA- AC079061.1. by CCK-8 and colony formation assays. F Confirmed the invasion and migration ability of lncRNA- AC079061.1. by transwell assays. G Compared the mRNA level of hsa-miR-765 and VIPR1 between si-lncRNA- AC079061.1 group and control group in MHCC97H and HCCLM3 cells. H Detected the protein levels of VIPR1 transfected with lncRNA- AC079061.1 siRNA or miR-765 mimic. I The luciferase activity of wildtype-lncRNA-AC079061.1 or mutant-lncRNA- AC079061.1 (wildtype- VIPR1 or mutant-VIPR1) after co-transfection with miR-765 mimic in MHCC97H and HCCLM3 cells; Putative binding sites between miR-765 and lncRNA- AC079061.1 or VIPR1 were predicted via lncBase v2.0. J, K CCK-8, transwell migration, and invasion assays were performed in MHCC97H cells treated with si NC + miR-NC, si lncRNA-AC079061.1 + miR-NC, si lncRNA-AC079061.1 + miR-765 inhibitor, si NC + miR-765 inhibitor. *P < 0.05, **P < 0.01, ***P < 0.001