Fig. 3

Syngeneic and allogenic, but not xenogeneic CPC transplantation improves cardiac recovery in the rat MI model. For the in vivo study, one million rCPCs prepared from Wister Kyoto (WK) rat heats were injected intramyocardially immediately after MI in Wister Kyoto (syngenic) and Brown Norway (allogenic) rats. The rats were followed for 4 weeks. Evaluation of left ventricular (LV) ejection fraction (A), fractional shortening (B), LV end-systolic volume (C), and cardiac output (D) by echocardiography on day 28. Images (E) and percent scar size (F) from Masson trichrome staining. Myocardial sections were evaluated at 28 days post-transplantation for angiogenesis by co-staining for IB4 and SMA (G–I), and cardiomyocyte proliferation for pHH3/SA (J, K). Hearts were harvested at day 28 post cell transplantation and evaluated for local inflammatory response by histology of stained sections (L) and inflammatory responses were scored in blinded manner (M). Systemic and humoral immune responses were evaluated by measuring circulating anti-donor IgM and IgG in serum (N). Numerical data are summarized as box and whisker plots with a median value (black bar inside box), 25th and 75th percentiles (bottom and top of box, respectively), and minimum and maximum values (bottom and top whisker, respectively). The number (n) of rats in each group indicated near the (up/below/on) each respective box and whisker plot