Fig. 6

The LINC00665/miR-582-5p/UCHL3 axis affected the radiosensitivity of NSCLC cells by modulating the stability of AhR protein. A, Western blot analysis of AhR protein level in A549 cells treated with sh-LINC00665 or combined with LV-AhR or in PC9 cells treated with LV-LINC00665 or combined with sh-AhR. B, Cell viability in A549 cells treated with sh-LINC00665 or combined with LV-AhR or in PC9 cells treated with LV-LINC00665 or combined with sh-AhR determined by CCK-8 assay. C, Colony formation potential of A549 cells treated with sh-LINC00665 or combined with LV-AhR or in PC9 cells treated with LV-LINC00665 or combined with sh-AhR determined by colony formation assay. D, Cell invasion in A549 cells treated with sh-LINC00665 or combined with LV-AhR or in PC9 cells treated with LV-LINC00665 or combined with sh-AhR determined by Transwell assay. E, Detection of γH2ax fluorescence in irradiation-treated A549 cells treated with sh-LINC00665 or combined with LV-AhR or in PC9 cells treated with LV-LINC00665 or combined with sh-AhR. F, Flow cytometry analysis of cell apoptosis in A549 cells treated with sh-LINC00665 or combined with LV-AhR or in PC9 cells treated with LV-LINC00665 or combined with sh-AhR. G, Flow cytometry analysis of cell cycle distribution in A549 cells treated with sh-LINC00665 or combined with LV-AhR or in PC9 cells treated with LV-LINC00665 or combined with sh-AhR. * p < 0.05 versus the A549 cells treated with sh-LINC00665 + Vector; # p < 0.05 versus the PC9 cells treated with LV-LINC00665 + sh-NC. Each cellular experiment was repeated 3 times