Table 1 Primers sequence
Gene name | Primer sequence (5’→3’) |
|---|---|
miR-32 | CGCGCTATTGCACATTACTAAGTTGCA |
Mef2d-F | GGCTGGCACTAGGCAATGTCAC |
Mef2d-R | CTGCTGTGGCTGTGGCTGTG |
iNOS-F | ACTCAGCCAAGCCCTCACCTAC |
iNOS-R | TCCAATCTCTGCCTATCCGTCTCG |
TNFÉ‘-F | GCGACGTGGAACTGGCAGAAG |
TNFÉ‘-R | GCCACAAGCAGGAATGAGAAGAGG |
p62-F | AGGAGGAGACGATGACTGGACAC |
p62-R | TTGGTCTGTAGGAGCCTGGTGAG |
Atg5-F | TGCGGTTGAGGCTCACTTTATGTC |
Atg5-R | GTCCCATCCAGAGCTGCTTGTG |
Becn-1-F | AGGCAGTGGCGGCTCCTATTC |
Becn-1-R | TGAGGACACCCAGGCAAGACC |
Atg16l1-F | CAAGCCGAATCTGGACTGTGGATG |
Atg16l1-R | CGGTCGTGACTTCCTGAGACAATC |
Runx2-F | AGTCCCAACTTCCTGTGCT |
Runx2-R | CTGCTCCGTTCTCAAAGTGG |
Bglap1-F | CAAGCAGGAGGGCAATAAGGTAGTG |
Bglap1-R | CATACTGGTCTGATAGCTCGTCACAAG |
Alp-F | CACGGCGTCCATGAGCAGAAC |
Alp-R | CAGGCACAGTGGTCAAGGTTGG |
SM22É‘-F | GCCTGAGAACCCACCCT |
SM22É‘-R | CGAAACCCGTCAAACCG |
αSMA-F | GGCATCCACGAAACCACCTAT |
αSMA-R | ATGAGACAGACCTAGCCACCG |
GAPDH-F | TGTTTCCTCGTCCCGTAG |
GAPDH-R | CAATCTCCACTTTGCCACT |