Fig. 4

Mef2d antagonises the function of miR-32 in VSMC osteogenic differentiation. A qRT-PCR analyzed the autophagy and osteogenic differentiation of VSMC after si-Mef2d transfection. B qRT-PCR analyzed the autophagy and osteogenic differentiation of VSMC after pDoubleEx-Mef2d transfection. C qRT-PCR analyzed the antagonism function of Mef2d for miR-32 in VSMC after transfected with pDoubleEx-MEF2D and miR-32 mimics. H Western blotting analyzed the autophagy and osteogenic differentiation of VSMC after transfected with si-Mef2d or pDoubleEx-MEF2D or pDoubleEx-MEF2D and miR-32 mimics, and statistical analysis (E–G). The experiment was repeated 3 times (n = 3), *p < 0.05 and **p < 0.01. Data was shown as means ± s.e.m