Fig. 3

miR-32 promotes VSMC osteogenic differentiation maybe through targeting Mef2d. A QRT-PCR analyzed the autophagy and osteogenic differentiation of VSMC after miR-32 mimics transfection. B, C Western-blotting analyzed the autophagy and osteogenic differentiation oa VSMC after miR-32 mimics transfection (B), and statistical analysis (C). D Venn diagram analyzed the target gene of miR-32 via miRDB database and Gene Ontology database. B Luciferase assay analyzed the combination of miR-32 and Mef2d 3’-UTR. F Immunohistochemistry analyzed MEF2D expression in WT and miR-32^−/− mice. Aorta from 3 mice in the two groups were dissected and were used to immunohistochemical analysis, respectively. The cells experiment was repeated 3 times (n = 3), *p < 0.05 and **p < 0.01. Data was shown as means ± s.e.m