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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Extracellular vesicle miR-32 derived from macrophage promotes arterial calcification in mice with type 2 diabetes via inhibiting VSMC autophagy

Fig. 1

High-glucose condition promotes macrophage secreting miR-32 EVs. A, B Flow cytometry analyzed the influence of high-glucose for M1 macrophage polarization (A), and statistical analysis (B). C qRT-PCR analyzed the influence of high-glucose for miR-32 expression in macrophage and EVs. D NTA analyzed the quantity and diameter of EVs. E Western blotting identified the expression of EVs marker protein, TSG101 and CD63. F Transmission electron microscope analysis EVs morphology. G Confocal microscopy observed VSMC engulfed macrophage EVs, which were stained with red fluorescence. The experiment was repeated 3 times (n = 3), *p < 0.05 and **p < 0.01. Data was shown as means ± s.e.m

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Journal of Translational Medicine

ISSN: 1479-5876

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