Fig. 2

Rhein reduced Ang II-induced CM hypertrophy and oxidative stress. A The 24-h exposure of CMs to rhein (0, 5, 10, 25, 50, and 100 μM). Cellular viability was examined by CCK-8 assay (n = 5 per experiment). B Immunoblots and quantification of ANP and BNP expression in CMs induced with Ang II and treated with rhein for 24 h. C Representative micrographs of CMs stained with α-actinin (red) and DAPI (blue) (scale bars = 20 μm; n ≥ 50 cells per group). D DCFH-DA detection of ROS (n = 5 per group). E, SOD and GSH activity (n = 5 per group). F, RT-qPCR quantification of NOX2 and NOX4 mRNA expression levels (n = 5 per experiment). *P < 0.05 and **P < 0.01 vs. the control group; ^#P < 0.05 and ^##P < 0.01 vs. the Ang II group